High-Throughput Immunoblotting Identifies Biotin-Dependent Signaling Proteins in HepG2 Hepatocarcinoma Cells
نویسندگان
چکیده
منابع مشابه
High-throughput immunoblotting identifies biotin-dependent signaling proteins in HepG2 hepatocarcinoma cells.
Biotin affects the abundance of mRNA coding for approximately 10% of genes expressed in human-derived hepatocarcinoma (HepG2) cells. Here, we determined whether effects of biotin on gene expression are associated with changes in the abundance of distinct proteins in cell signaling and structure. HepG2 cells were cultured in media containing the following concentrations of biotin: 0.025 nmol/L (...
متن کاملHigh-Throughput Immunoblotting Identifies Biotin-Dependent Signaling Proteins in HepG2 Hepatocarcinoma Cells1
Biotin affects the abundance of mRNA coding for 10% of genes expressed in human-derived hepatocarcinoma (HepG2) cells. Here, we determined whether effects of biotin on gene expression are associated with changes in the abundance of distinct proteins in cell signaling and structure. HepG2 cells were cultured in media containing the following concentrations of biotin: 0.025 nmol/L (denoted “defic...
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Retinoids have shown clinical efficacy in cancer chemoprevention and therapy presumably by modulating the growth, differentiation, and apoptosis of normal, premalignant, and malignant cells. To better understand the mechanisms by which retinoids exert their effects, we used a high-throughput Western blotting method (Becton-Dickinson PowerBlot) to evaluate changes in the levels of cellular signa...
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ژورنال
عنوان ژورنال: The Journal of Nutrition
سال: 2005
ISSN: 0022-3166,1541-6100
DOI: 10.1093/jn/135.7.1659